Table 2.
Functional categories of predicted open reading frames (ORFs) in bacteriophage SA97.
| Group | Subgroup | Locus_tag a | Function | BLASTP Best Match | Identity (%) b | Accession no. c |
|---|---|---|---|---|---|---|
| packaging | SA97_010 | terminase small subunit | phage terminase small subunit [Staphylococcus aureus subsp. aureus JH1] | 164/164 (100) | YP_001316047.1 | |
| packaging | SA97_011 | terminase large subunit | PBSX family phage terminase large subunit [Staphylococcus aureus subsp. aureus JH9] | 431/431 (100) | YP_001246264.1 | |
| packaging | SA97_012 | portal protein | SPP1 family phage portal protein [Staphylococcus aureus subsp. aureus JH9] | 512/512 (100) | YP_001246265.1 | |
| structure | head | SA97_013 | head morphogenesis protein | phage head morphogenesis protein [Staphylococcus aureus subsp. aureus JH9] | 331/331 (100) | YP_001246266.1 |
| hypothetical | SA97_014 | hypothetical protein | hypothetical protein phiETA2_gp45 [Staphylococcus phage phiETA2] | 56/56 (100) | YP_001004305.1 | |
| structure | head | SA97_015 | capsid protein | phage capsid protein [Staphylococcus aureus] | 206/206 (100) | WP_000392146.1 |
| structure | head | SA97_016 | major capsid protein | Phage major capsid protein [Staphylococcus aureus subsp. aureus DSM 20231] | 324/324 (100) | WP_001836233.1 |
| hypothetical | SA97_017 | hypothetical protein | phage protein [Staphylococcus aureus subsp. aureus DSM 20231] | 95/95 (100) | ELP28728.1 | |
| structure | head-tail | SA97_018 | head-tail adapter protein | head-tail connector protein [Staphylococcus phage SA12] | 110/110 (100) | YP_008241892.1 |
| hypothetical | SA97_019 | hypothetical protein | hypothetical protein NWMN_1023 [Staphylococcus aureus subsp. aureus str. Newman] | 115/115 (100) | YP_001332057.1 | |
| structure | head | SA97_020 | head protein | hypothetical protein SAV0897 [Staphylococcus aureus subsp. aureus Mu50] | 127/127 (100) | NP_371421.1 |
| structure | tail | SA97_021 | major tail protein | phage major tail protein [Staphylococcus phage phiNM] | 193/193 (100) | YP_873998.1 |
| hypothetical | SA97_022 | hypothetical protein | hypothetical protein SaurJH9_0899 [Staphylococcus aureus subsp. aureus JH9] | 121/121 (100) | YP_001246276.1 | |
| hypothetical | SA97_023 | hypothetical protein | hypothetical protein T874_02808, partial [Staphylococcus aureus SJOS6126] | 114/114 (100) | EVG26589.1 | |
| structure | tail | SA97_024 | tape measure protein | putative tape measure protein [Staphylococcus phage phiSauS-IPLA88] | 1130/1154 (98) | YP_002332525.1 |
| structure | tail | SA97_025 | putative minor tail protein | putative minor tail protein [Staphylococcus phage TEM123] | 314/315 (99) | YP_ 006382275.1 |
| structure | tail | SA97_026 | prophage endopeptidase tail protein | SGNH hydrolase superfamily [Staphylococcus aureus subsp. aureus 21283] | 632/632 (100) | EHO98287.1 |
| structure | tail | SA97_027 | putative minor tail protein | minor structural protein [Staphylococcus aureus] | 635/636 (99) | WP_000369023.1 |
| hypothetical | SA97_028 | hypothetical protein | hypothetical protein NWMN_0306 [Staphylococcus aureus subsp. aureus str. Newman] | 607/607 (100) | YP_001331340.1 | |
| hypothetical | SA97_029 | hypothetical protein | SLT orf 129-like protein [Staphylococcus aureus subsp. aureus JH1] | 125/125 (100) | YP_001316067.1 | |
| hypothetical | SA97_030 | hypothetical protein | hypothetical protein SAP26_gp20 [Staphylococcus phage SAP-26] | 57/57 (100) | YP_003857088.1 | |
| hypothetical | SA97_031 | hypothetical protein | hypothetical protein phiETA2_gp63 [Staphylococcus phage phiETA2] | 99/99 (100) | YP_001004323.1 | |
| structure | tail | SA97_032 | mannosyl-glycoprotein endo-beta-N-acetylglucosminidase | mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase [Staphylococcus aureus subsp. aureus DSM 20231] | 632/632 (100) | WP_001836236.1 |
| structure | tail | SA97_033 | putative tail fiber protein | tail fiber [Staphylococcus aureus subsp. aureus JH1] | 412/412 (100) | YP_001316071.1 |
| hypothetical | SA97_034 | hypothetical protein | hypothetical protein SASA_03740 [Staphylococcus aureus subsp. aureus DSM 20231] | 131/131 (100) | ELP28745.1 | |
| host lysis | SA97_035 | holin | holin, SPP1 family [Staphylococcus aureus subsp. aureus 21267] | 91/91 (100) | EZH90125.1 | |
| host lysis | SA97_036 | amidase | CHAP domain-containing protein [Staphylococcus aureus subsp. aureus JH9] | 470/470 (100) | YP_001246290.1 | |
| hypothetical | SA97_037 | hypothetical protein | hypothetical protein SaurJH1_0932 [Staphylococcus aureus subsp. aureus JH1] | 185/185 (100) | YP_001316075.1 | |
| hypothetical | SA97_038 | hypothetical protein | hypothetical protein SAOUHSC_02018 [Staphylococcus aureus subsp. aureus NCTC 8325] | 50/50 (100) | YP_500515.1 | |
| regulation | SA97_039 | putative transcriptional regulatory protein | XRE family transcriptional regulator [Staphylococcus aureus] | 61/61 (100) | WP_000382163.1 | |
| lysis/lysogeny | SA97_040 | putative integrase | integrase [Staphylococcus aureus subsp. aureus DSM 20231] | 349/349 (100) | WP_001145725.1 | |
| hypothetical | SA97_041 | hypothetical protein | hypothetical protein SAOUHSC_02087 [Staphylococcus aureus subsp. aureus NCTC 8325] | 301/301 (100) | YP_500579.1 | |
| hypothetical | SA97_042 | hypothetical protein | hypothetical protein phiETA2_gp04 [Staphylococcus phage phiETA2] | 241/241 (100) | YP_001004264.1 | |
| hypothetical | SA97_043 | hypothetical protein | hypothetical protein SaurJH1_0868 [Staphylococcus aureus subsp. aureus JH1] | 224/224 (100) | YP_001316011.1 | |
| regulation | SA97_044 | putative transcriptional regulator | XRE family transcriptional regulator [Staphylococcus aureus] | 98/110 (89) | WP_001055143.1 | |
| lysis/lysogeny | SA97_045 | anti-repressor | anti-repressor protein [Staphylococcus phage phiETA2] | 255/255 (100) | YP_001004268.1 | |
| hypothetical | SA97_046 | hypothetical protein | hypothetical protein WWS_01005 [Staphylococcus aureus M1510] | 154/155 (99) | ENN21654.1| | |
| hypothetical | SA97_047 | hypothetical protein | hypothetical protein SAV0856 [Staphylococcus aureus subsp. aureus Mu50] | 74/74 (100) | NP_371380.1 | |
| hypothetical | SA97_048 | hypothetical protein | hypothetical protein phiETA2_gp10 [Staphylococcus phage phiETA2] | 148/149 (99) | YP_001004270.1 | |
| hypothetical | SA97_049 | hypothetical protein | hypothetical protein phiETA3_gp11 [Staphylococcus phage phiETA3] | 73/73 (100) | YP_001004340.1 | |
| hypothetical | SA97_050 | hypothetical protein | hypothetical protein SaurJH1_0876 [Staphylococcus aureus subsp. aureus JH1] | 100/100 (100) | YP_001316019.1 | |
| hypothetical | SA97_051 | hypothetical protein | hypothetical protein SASA_03250 [Staphylococcus aureus subsp. aureus DSM 20231] | 86/86 (100) | ELP28696.1 | |
| hypothetical | SA97_052 | hypothetical protein | hypothetical protein phiSLTp16 [Staphylococcus phage phiSLT] | 177/178 (99) | NP_075479.1 | |
| regulation | SA97_053 | single-stranded DNA-binding protein 1 | ssDNA-binding protein St aphylococcus aureus SA_ST125_MupR] | 216/216 (100) | EPR23947.1 | |
| regulation | SA97_054 | single-stranded DNA-binding protein 2 | ssDNA-binding protein [Staphylococcus phage phiETA3] | 104/105 (99) | YP_001004346.1 | |
| hypothetical | SA97_001 | hypothetical protein | hypothetical protein U622_02779, partial [Staphylococcus aureus F53399] | 61/61 (100) | EWV07862.1 | |
| hypothetical | SA97_002 | hypothetical protein | hypothetical protein SaurJH9_0873 [Staphylococcus aureus subsp. aureus JH9] | 101/101 (100) | YP_001246250.1 | |
| hypothetical | SA97_003 | hypothetical protein | hypothetical protein phiETA3_gp28 [Staphylococcus phage phiETA3] | 118/118 (100) | YP_001004357.1 | |
| hypothetical | SA97_004 | hypothetical protein | hypothetical protein SASA_03390 [Staphylococcus aureus subsp. aureus DSM 20231] | 80/80 (100) | ELP28710.1 | |
| hypothetical | SA97_005 | hypothetical protein | phage protein [Staphylococcus aureus M0157] | 110/110 (100) | EUU37240.1 | |
| regulation | SA97_006 | dUTP diphosphatase | dUTP diphosphatase [Staphylococcus aureus A9765] | 175/176 (99) | WP_001797468.1 | |
| hypothetical | SA97_007 | hypothetical protein | hypothetical protein [Staphylococcus phage phiMR25] | 67/68 (99) | YP_001949833.1 | |
| regulation | SA97_008 | transcriptional activator RinB | transcriptional activator RinB [Staphylococcus aureus subsp. aureus CM05] | 57/57 (100) | WP_001657250.1 | |
| regulation | SA97_009 | transcriptional activator RinA | transcriptional activator RinA [Staphylococcus phage phiETA3] | 140/140 (100) | YP_001004368.1 |
a, Each predicted ORFs of SA97 phage was labeled with locus_tag. b, Identities were calculated between amino acid sequences. c, Genbank accession numbers.