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. 2015 Oct 5;7(10):5243–5256. doi: 10.3390/v7102871

Figure 1.

Figure 1

Cx43 and hDlg interact directly in cervical tumour cells in vivo. (A) Representative immunofluorescence image showing a section of a high grade cervical lesion; (B) A region of Cx43 and hDlg colocalisation in (A) enlarged 5×. Immunofluorescence shows hDlg (green), Cx43 (red) and DAPI (blue). The yellow arrow indicates one area of Cx43/hDlg colocalisation; (C) Immunofluorescence proximity ligation for hDlg and Cx43, where red staining indicates colocalisation of the proteins within 40 nm of each other; (D) Regions of Cx43 and hDlg colocalisation detected by PLA in C enlarged 5×; (E) Immunofluorescence proximity ligation for hDlg and Cx43 in a region of a cervical tumour; (F) Immunofluorescence proximity ligation secondary control on the outer region of the cervical lesion a part of which is shown in (C).