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. 2015 Oct 27;7(10):8871–8886. doi: 10.3390/nu7105437

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of (−)-Epigallocatechin-3-gallate (EGCG) on the expression of adipogenic transcription factors during adipogenesis. (A) EGCG decreased both C/EBPα and Peroxisome proliferator-activated receptor γ (PPARγ) protein expression levels in a dose-dependent manner. Changes in expression of PPARγ (B) and C/EBPα (C) in differentiated 3T3-L1 adipocytes treated with EGCG as analyzed by quantitative real-time polymerase chain reaction (PCR). Results were normalized to the expression of α-tubulin and are expressed as relative mRNA level compared with the average expression in cells incubated without EGCG. The error bars represent the standard deviation (SD) (n = 3); * p < 0.05, ** p < 0.01 vs. the control.