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. 2015 Oct 27;7(10):8871–8886. doi: 10.3390/nu7105437

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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The effects of (−)-Epigallocatechin-3-gallate (EGCG) on vascular endothelial growth factor (VEGF) expression in 3T3-L1 preadipocytes and adipocytes. VEGF mRNA levels of 3T3-L1 preadipocytes (A) and adipocytes (B) as quantified using 7000 Real-Time PCR System (Applied Biosystems); (C) VEGF concentrations in 3T3-L1 preadipocyte conditioned media which was treated with or without EGCG for 48 h as analyzed by enzyme linked immunosorbent assay (ELISA); (D) VEGF concentrations in the conditioned media from 3T3-L1 cells treated with or without EGCG during adipocyte differentiation as measured by ELISA. Data are expressed as a percentage of the control (non-treated cells). Values are expressed as mean ± standard deviation (SD), n = 3. * p < 0.05, ** p < 0.01 vs. the control.