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. 2015 Nov 3;21:44. doi: 10.1186/s40409-015-0044-5

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© Prinholato da Silva et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Assessment of cell cycle progression of the tumor cell lines HL-60 (a), HepG2 (b), PC-12 (c) and B16F10 (d) treated with BthTX-I using flow cytometry. Tumor cells were treated with BthTX-I at different concentrations (5 to 100 μg/mL). Cells treated with PBS were used as negative control (PBS). The cells were fixed with ethanol and stained with propidium iodide present in the HFS solution. G0/G1: resting/preparation for synthesis; S: DNA synthesis; G2/M: preparation for mitosis. Results expressed as mean ± SD of three independent experiments (n = 3). Statistically significant differences (p < 0.05) are labeled with * (in comparison with G0/G1 phase of PBS)