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. 2015 Jul 22;87(11):1038–1046. doi: 10.1002/cyto.a.22717

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© 2015 The Authors. Cytometry Part A Published by Wiley Periodicals, Inc.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Analysis of three donors indicate that data obtained from PSMS vs. PBMC generated compensation matrices are similar. A: Mean and standard deviation of negative and positive populations for each T cell panel marker using compensation matrices generated from PSMS and PBMC. B: X‐Median (Median fluorescence intensity) of three donors PBMC stained with panel specific markers given the compensation matrix generated by PSMS and PBMC. C: SI of PBMC from three donors using PSMS and PBMC matrices.