Table 3.

Stain index

	PSMS	PBMC
FL1: FITC 525/30 BP	50.8	14.1
FL2: PE 575/30 BP	44.4	22.3
FL3: ECD 620/30 BP	78.4	4.3
FL4: PERCP 670 LP	1.4	18.3
FL5: PE‐Cy7 775 LP	51.2	10.1
FL6: APC 660/20 BP	61.4	49.2
FL7: APC‐A700 725/20 BP	38.9	9.0
FL8: APC‐A750 775 LP	28.0	40.3
FL9: Pacific Blue 450/50 BP	28.9	89.9
FL10: Krome Orange 550/40 BP	20.1	24.8
$\text{SI} = \frac{(\text{median}\%\text{positive} - \text{median}\%\text{negative}) }{\left(2 \times \text{sd} \% \text{negative}\right)}$