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. 2015 Jul 22;5(7):140171. doi: 10.1098/rsob.140171

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© 2015 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 7. — Gain of JNK signalling promotes Drs expression and dorsal nuclear localization. (a–c) Fluorescent microscope images showing third-instar larvae. Compared with control (a), expression of dTAK1 (b) or RNAi inactivation of puc (c) in fat body upregulates Drs-GFP expression. (d) Fluorescent microscope images showing fat body dissected from third-instar larvae stained with anti-dorsal (red). dTAK1-expressing clones were tagged by GFP (green) and induced for 1 h by heat shock at 37°C and recovered for 24 h at 25°C. Nuclei were labelled with DAPI (blue). Endogenous Dorsal protein displays nuclear localization in cells expressing high level of dTAK1 (arrows). (d′) shows high magnification views of the boxed area in (d). DAPI, 4, 6-diamidino-2-phenylindole. See the electronic supplementary material for detailed genotypes.