Figure 8.

Gain of JNK signalling upregulates the expression of Spz family ligands. (a) Histogram showing the levels of Spz1–6 mRNAs measured by quantitative RT-PCR. Total RNA of Drosophila adult eyes was extracted and normalized for cDNA synthesis. Error bars represent standard deviation from three independent experiments. (b–f) Fluorescence micrographs of Drosophila third-instar larval eye discs are shown. Compared with the GMR-GAL4 control (c), Spz6-GFP expression was evidently increased by expression of Egr (d), dTAK1 (e) or Hep (f), mRFP marking the GMR-GAL4 expression region (b). The right panels show views of vertical cross sections corresponding to the left panels (b–f). Nuclei were labelled with DAPI (blue), cell membranes were stained by anti-Dlg antibody (red). Imaging of prepared samples was conducted by a Leica confocal microscope (Leica SP5). See the electronic supplementary material for detailed genotypes.