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Schematic representation of PknB constructs and strains generated in this study. Kinase, N-terminal kinase domain (aa 1–279); TM, transmembrane region (aa 331–354); TM-PASTA, penicillin and serine or threonine kinase-associated domain (aa 331–627); MYC-HIS, His-Tag; PASTA (aa 354–627).
