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Effect of pknB and pknB-ΔPASTA overexpression on growth of M. smegmatis. Mycobacteria (5 × 106) from early stationary phase were inoculated in 100-well honeycomb plates, containing various media and incubated in Bioscreen Growth analyser at 37°C with shaking for 5 days in the presence of tetracycline. Presented data are mean values of 5 replicates from two independent experiments. Standard deviations were 10% or less of average values and are not shown for clarity.
