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. 2015 Jul 1;5(7):150025. doi: 10.1098/rsob.150025

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© 2015 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 4. — Overexpression of PknB_PASTA results in alteration of bacterial morphology and vancomycin labelling. Mycobacteria from logarithmic growth phase were labelled with Vancomycin-BODIPY conjugate, washed and mounted in PBS and examined using a Diphot 300 inverted microscope. Three independent fields for each strain are shown. Bars indicate 5 μm. (a) MIND and (b) TMP. Left panel, phase contrast; middle panel, fluorescence microscopy; right panel, merged images. Arrows indicate labelled peptidoglycan.