Table 1.

Module plasmids created in this study. Systematic numbers (denoted with # throughout the manuscript) and plasmid names are listed with module contents. Each module was PCR-amplified from the indicated origin template to add particular cohesive ends, the names of which are listed in ‘names of cohesive ends’ (a–g shown in figure 2c and table 2). The amplified module fragment was cloned into pCR-Blunt II-TOPO. The vector name pBMod indicates pCR-Blunt II-TOPO-based module plasmid. An Excel version of this table is available in the electronic supplementary material.

	module plasmids		modules (contents)	name of cohesive ends (left, right)	origin	literature	notes
	number	name
	I. Promoter (ab)
figures 2 and 3	1	pBMod-Padh1(ab)	Padh1	a, b	S. pombe genome	Russell & Hall [13]
	2	pBMod-Padh41(ab)	Padh41	a, b	Padh1, mutagenesis	Yamagishi et al. [14]
	3	pBMod-Padh81(ab)	Padh81	a, b	Padh1, mutagenesis	Yokobayashi & Watanabe [2]
	4	pBMod-Pnmt1(ab)	Pnmt1	a, b	S. pombe genome	Maundrell [15]
	5	pBMod-Pnmt41(ab)	Pnmt41	a, b	Pnmt1, mutagenesis	Basi et al. [16]
	6	pBMod-Pnmt81(ab)	Pnmt81	a, b	Pnmt1, mutagenesis	Basi et al. [16]
	7	pBMod-Purg1(ab)	Purg1	a, b	pFA6a-kanMX6-Purg1	Watt et al. [17]
	III. FPtag-C (cd)
figure 2	8	pBMod-L-GFP(cd)	L-GFPa	c, d	pFA6a-GFP(S65T)-kanMX	Bähler et al. [12]	BsaI removed
(C tag)	9	pBMod-L-mCherry(cd)	L-mCherrya	c, d	pFA6a-mCherry-hphMX	Sato et al. [18]
	10	pBMod-L-ECFP(cd)	L-ECFPa	c, d	pFA6a-ECFP-natMX	Sato et al. [18]
	IIIa. FPcontrol-C (bd)
	11	pBMod-cont-L-GFP(bd)	control L-GFPa	b, d	pFA6a-GFP(S65T)-kanMX	Bähler et al. [12]	BsaI removed
	12	pBMod-cont-L-mCherry(bd)	control L-mCherrya	b, d	pFA6a-mCherry-hphMX	Sato et al. [18]
	13	pBMod-cont-L-ECFP(bd)	control L-ECFPa	b, d	pFA6a-ECFP-natMX	Sato et al. [18]
figure 3	VII. FPtag-N (bc)
(N tag)	14	pBMod-GFP-L(bc)	GFP-La	b, c	pFA6a-GFP(S65T)-kanMX	Bähler et al. [12]	BsaI removed
	15	pBMod-mCherry-L(bc)	mCherry-La	b, c	pFA6a-mCherry-hphMX	Sato et al. [18]
	16	pBMod-ECFP-L(bc)	ECFP-La	b, c	pFA6a-ECFP-natMX	Sato et al. [18]
	VIIa. FPcontrol-N (bd)
	17	pBMod-cont-GFP-L(bd)	control GFP-La	b, d	pFA6a-GFP(S65T)-kanMX	Bähler et al. [12]	BsaI removed
	18	pBMod-cont-mCherry-L(bd)	control mCherry-La	b, d	pFA6a-mCherry-hphMX	Sato et al. [18]
	19	pBMod-cont-ECFP-L(bd)	control ECFP-La	b, d	pFA6a-ECFP-natMX	Sato et al. [18]
figures 2 and 3	IV. Terminator + Marker (de)
	20	pBMod-Tadh-kan(de)	Tadh-kan	d, e	pFA6a-GFP(S65 T)-kanMX	Bähler et al. [12]
	21	pBMod-Tadh-hph(de)	Tadh-hph	d, e	pFA6a-mCherry-hphMX	Sato et al. [18]
	22	pBMod-Tadh-nat(de)	Tadh-nat	d, e	pFA6a-ECFP-natMX	Sato et al. [18]
	23	pBMod-Tadh-bsd(de)	Tadh-bsd	d, e	pCR2.1-bsd	Kimura et al. [19]
	IVa. Terminator (dg)
	24	pBMod-Tadh(dg)	Tadh	d, g	pFA6a-GFP(S65 T)-kanMX	Bähler et al. [12]
	IVb. Marker (ge)
	25	pBMod-kan(ge)	kan	g, e	pFA6a-GFP(S65 T)-kanMX	Bähler et al. [12]
	26	pBMod-hph(ge)	hph	g, e	pFA6a-mCherry-hphMX	Sato et al. [18]
	27	pBMod-nat(ge)	nat	g, e	pFA6a-ECFP-natMX	Sato et al. [18]
	28	pBMod-bsd(ge)	bsd	g, e	pCR2.1-bsd	Kimura et al. [19]
	29	pBMod-ura4 + (ge)	ura4+	g, e	pREP2	Maundrell [15]
	30	pBMod-LEU2(ge)	LEU2	g, e	pREP1	Maundrell [15]
figures 2 and 3	V. Target (ef)
	31	pBMod-co2(ef)	co2	e, f	S. pombe genome	this study
	32	pBMod-Z2(ef)	Z2	e, f	S. pombe genome	Akera et al. [20]
	33	pBMod-lys1(ef)	lys1	e, f	S. pombe genome	pombase.org
	34	pBMod-arg1(ef)	arg1	e, f	S. pombe genome	pombase.org
figures 2 and 3	VI. Vector (fa)
	35	pBMod-AmpVec(fa)	AmpR vector	f, a	pFA6a	Wach et al. [21]	BsaI removed, lacking a 137 bp fragment (see Material and methods for details)

^aL, linker.