Figure 2.

Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of different horseradish peroxidase (HRP) variants. Black boxes indicate protein bands analyzed by mass spectrometry. Lane 1, BLUeye Prestained Protein Ladder; lanes 2 and 3, HRP from plant in two different concentrations (4 μg protein and 8 μg protein, respectively); lane 4, wt^{wt HRP}, unmutated HRP enzyme expressed in the benchmark strain; lane 5, wt^{4/8 HRP}, mutated 4/8 HRP variant expressed in the benchmark strain; lane 6, OCH1^{4/8 HRP}, mutated 4/8 HRP variant expressed in the deleted OCH1 gene strain.