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. 2015 Sep 30;16(10):23572–23586. doi: 10.3390/ijms161023572

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Agarose gel electrophoresis to confirm the plasmid pAZI9479 and the PCR product of asadhʹ. (A) Lane 1, λ DNA/Hind III marker; Lane 2, plasmid pAZI9479; (B) Lane 1, DL2000 marker; Lane 2, asadhʹ PCR product released by diagnostic restriction enzyme (Nco I and Nde I) digestion.