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. 2015 Sep 30;16(10):23572–23586. doi: 10.3390/ijms161023572

Figure 4.

Figure 4

Western blot analysis of the relationship between ASADH expression and the concentration of pristinamycin over time (20 μg of total protein were loaded for cataphoresis). Lane 1, EasySee Western Marker; Lane 2 starved mutant; Lanes 3–8, ASADH expression on days 1, 3, 5, 7, 9, and 11 at 10−1 µg/mL; Lanes 9–14, ASADH expression on days 1, 3, 5, 7, 9, and 11 at 10−5 µg/mL. The experiments were repeated three times, and typical images are shown.