Figure 5.

The proportion of the mass_cell-wall to the mass_whole-cell. The whole cell wall was extracted from wild-type H37Rv and MTB::asadh bacteria cultured in the presence of 10⁻¹, 10⁻⁵ and 0 µg/mL pristinamycin. (A) The proportion of the mass_cell-wall (M_cell-wall) to the mass_bioplasm (M_bioplasm) in the wild-type and MTB::asadh bacteria cultured in the presence of pristinamycin was approximately 140%, compared with approximately 30% in the MTB::asadh bacteria cultured in the absence of pristinamycin; (B) The proportion of the mass_cell-wall to the mass_whole-cell in the wild-type and MTB::asadh bacteria cultured in the presence pristinamycin was approximately 60%, compared with 25% in the MTB::asadh cells cultured in the absence of pristinamycin. The experiments were repeated three times, and results were expressed as mean ± SD. Results were compared using one-way ANOVA method (* p < 0.05).