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. 2015 Sep 30;16(10):23587–23603. doi: 10.3390/ijms161023587

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Affinity-matured monobodies bound specifically to their targets in enzyme-linked immunosorbent assay (ELISA). Virions displaying affinity-matured monobodies binding to mitogen-activated protein kinase kinase 5 (2C12) and ubiquitin carboxyl-terminal hydrolase 11 (1C2) were tested in phage ELISA for specificity against a panel of nine other biotinylated targets. NeutrAvidin was used to immobilize the targets to the well. Phage particles bound to the target were detected via anti-M13-horseradish peroxidase (HRP) antibody. Both clones specifically recognized their cognate targets.