. 2015 Sep 30;16(10):23587–23603. doi: 10.3390/ijms161023587

Table 2.

Output sequences and affinities of clones after affinity maturation.

Targets	Clones	BC Loop (26–30)	FG Loop (77–81)	Framework Mutations	Affinity (nM)
Targets	Clones	BC Loop (26–30)	FG Loop (77–81)	Framework Mutations	ELISA	ITC
CDC34	1D10	CGLWC	PGLRL	S17I, T76I	<50	N/D
COPS5	1D7	RRWDV	WGIII	None	<10	N/D
MAP2K5	1C4	CRKCL	RLEWL	P51H, K83N	6	11
MAP2K5	2C12	CRKCL	RLEFL	None	17	6
SF3A1	1E2	ALPVY	VWWYE	None	<50	N/D
USP11	1C2	WWVPQ	PGIYQ	L18M, G61C, G65D, S82I	N/D	4
USP11	1A9	WWSVP	PGIYA	D67V, S82I, Y92C	52	N/D

BC and FG loops are the variable regions. Estimated affinities were determined by competition; phage ELISA and/or isothermal titration calorimetry (ITC). N/D: experiments were not; performed to determine the values.