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. 2015 Oct 2;16(10):23615–23629. doi: 10.3390/ijms161023615

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Western blot analysis of IGF-2 protein in the SW620 human colon adenocarcinoma cell line. Cells were exposed to 3 mM 5-ALA or met-ALA and additionally treated with light (4.5 J/cm²) or not irradiated. Differences between samples were found: Lane 1 shows the band from control cells without treatment; Lane 2 from cells treated with light only; Lanes 3 and 5 from cells incubated with 5-ALA or met-ALA, respectively; Lanes 4 and 6 from cells treated with PDT at the above doses. An increase in IGF-2 was also observed in cells treated with light only (Lane 2) and photosensitizer precursors without light irradiation (Lanes 3 and 5). M, Marker mass.