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. 2015 Oct 9;16(10):23823–23848. doi: 10.3390/ijms161023823

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

The effects of p-β-catenin Y333 knockdown or overexpression on the migration, invasion, and MMP expression and activity of U87 cells. The effects of p-β-catenin on the malignant biological behavior of glioma cells were investigated by establishing stably transfected U87 cells with p-β-catenin Y333 silencing or overexpression plasmids. p-β-catenin Y333 knockdown attenuated the migration, invasion, and MMP expression and activity in U87 cells, whereas its overexpression enhanced the malignant biological behavior of glioma cells. (A) The knockdown transfection efficiency. The expression of p-β-catenin Y333 protein was checked by Western blot assays. p-β-catenin Y333 was significantly inhibited in the shRNA-p-β-catenin Y333 group individually compared with the control group. The NC control group showed no obvious difference to the control group; (B) The overexpression transfection efficiency. P-β-catenin Y333 was significantly upregulated in the pIRES2-p-β-catenin Y333 group individually compared with the control group. The NC control group showed no obvious difference with the control group; (C) Results of Transwell in vitro migration assay. The migration of U87 cells was either inhibited or promoted by p-β-catenin Y333 knockdown or overexpression; (D) Results of Transwell in vitro invasion assay. The invasion of U87 cells was either inhibited or promoted by p-β-catenin Y333 knockdown or overexpression; (E) The effects of p-β-cateninY333 knockdown or overexpression on the MMP expression of U87 cells assessed by Western blot assay. The expression of MMP-2 and MMP-9 was either inhibited or promoted by p-β-cateni nY333 knockdown or overexpression; (F) The effects of p-β-cateninY333 knockdown or overexpression on the MMP-2 and MMP-9 activity of U87 cells. The activity of MMP-2 and MMP-9 was inhibited by p-β-catenin Y333 knockdown but promoted by p-β-catenin Y333 over-expression. * p < 0.05, ** p < 0.01 compared with control (n = 5). Bar stands for 50 μm. Original magnification of A,C: ×400.

The effects of p-β-catenin Y333 knockdown or overexpression on the migration, invasion, and MMP expression and activity of U87 cells. The effects of p-β-catenin on the malignant biological behavior of glioma cells were investigated by establishing stably transfected U87 cells with p-β-catenin Y333 silencing or overexpression plasmids. p-β-catenin Y333 knockdown attenuated the migration, invasion, and MMP expression and activity in U87 cells, whereas its overexpression enhanced the malignant biological behavior of glioma cells. (A) The knockdown transfection efficiency. The expression of p-β-catenin Y333 protein was checked by Western blot assays. p-β-catenin Y333 was significantly inhibited in the shRNA-p-β-catenin Y333 group individually compared with the control group. The NC control group showed no obvious difference to the control group; (B) The overexpression transfection efficiency. P-β-catenin Y333 was significantly upregulated in the pIRES2-p-β-catenin Y333 group individually compared with the control group. The NC control group showed no obvious difference with the control group; (C) Results of Transwell in vitro migration assay. The migration of U87 cells was either inhibited or promoted by p-β-catenin Y333 knockdown or overexpression; (D) Results of Transwell in vitro invasion assay. The invasion of U87 cells was either inhibited or promoted by p-β-catenin Y333 knockdown or overexpression; (E) The effects of p-β-cateninY333 knockdown or overexpression on the MMP expression of U87 cells assessed by Western blot assay. The expression of MMP-2 and MMP-9 was either inhibited or promoted by p-β-cateni nY333 knockdown or overexpression; (F) The effects of p-β-cateninY333 knockdown or overexpression on the MMP-2 and MMP-9 activity of U87 cells. The activity of MMP-2 and MMP-9 was inhibited by p-β-catenin Y333 knockdown but promoted by p-β-catenin Y333 over-expression. * p < 0.05, ** p < 0.01 compared with control (n = 5). Bar stands for 50 μm. Original magnification of A,C: ×400.