Table 1.

Pathogen-secreted proteins of vascular wilt fungi.

Protein Name	Pathogen	Virulence Phenotype Deletion Mutant	Avr 1	Comments	Reference
Small, Secreted Proteins
Six1	Fol	reduced virulence on tomato	yes (I-3)		[23]
Six2	Fol				[23]
Six3	Fol	reduced virulence on tomato	yes (I-2)	Interacts with Six5	[23]
Six4	Fol	no suppression of I-2/I-3-mediated resistance on tomato	yes (I, I-1)		[23]
Six4	Fo5176	reduced virulence on At			[24]
Six4	Foc	reduced virulence on cabbage			[25]
Six5	Fol	reduced virulence on tomato		Interacts with Six3 Required for I-2-mediated immunity	[6]
Six6	Fol	reduced virulence on tomato			[6]
Six7	Fol				[6]
Six8	Fol			Multi-copy gene in Fol	[6]
Six8b	Fol			Multi-copy gene in Fol	[6]
Six9-14	Fol				[6]
VDAG_05180	Vd VdLs17	reduced virulence on tomato		Two LysM domains	[9]
Ave1	Vd JR2	reduced virulence on tomato	yes (Ve1)	Homology to PNPs	[26]
Ave1	Fol		yes (Ve1)	Not found in xylem during infection	[26]
XLOC_009059	Vd JR2	reduced virulence on tomato			[9]
XLOC_008951	Vd JR2	reduced virulence on tomato			[9]
NEP (-like) Proteins
NEP1	Foe	no virulence effect on coca		Ethylene and necrosis-inducing factor on several dicotylous plants	[27,28]
NEP	Vd-8			Wilt-inducing factor on cotton	[29]
NLP1	Vd V592	no virulence effect on cotton		Wilt- and necrosis-inducing factor on cotton	[30]
NLP2	Vd V592	no virulence effect on cotton		Wilt- and necrosis-inducing factor on cotton	[30]
NLP3-9	Vd V592				[30]
NLP1	Vd JR2	reduced virulence on tomato, At and Nb		Necrosis-inducing factor on Nb; Mutant has reduced conidiophore formation and extensive formation of aerial hyphae	[31]
NLP2	Vd JR2	reduced virulence on tomato and At		Necrosis-inducing factor on Nb	[31]
NLP3-9	Vd JR2				[31]
Secreted Enzymes
PG1	Fol	Δpg1Δpgx6 double mutant reduced virulence on tomato			[32]
PGX6	Fol	Δpg1Δpgx6 double mutant reduced virulence on tomato			[32]
TOM1	Fol	reduced virulence on tomato		Tomatinase activity ~25% reduced in deletion mutant	[33]
Mep1	Fol	Δfomep1Δfosep1 double mutant reduced virulence on tomato			[34]
Sep1	Fol	Δfomep1Δfosep1 double mutant reduced virulence on tomato			[34]
Isc1	Vd V991	reduced virulence on cotton			[35]
Hydrophobins
Cerato-ulmin	Onu	no virulence effect on elm			[36]
VDH1	Vd Dvd-T5	no virulence effect on tomato		Reduced microsclerotia production in deletion mutant	[37]

Six = secreted in xylem; NEP = necrosis- and ethylene-inducing protein; NLP = NEP-like protein; PG = endopolygalacturonase; PGX = exopolygalacturonase; TOM = tomatinase; Mep = metalloprotease; Sep = serine protease; Isc = isochorismatase; Fol = Fusarium oxysporum f. sp. lycopersici; Fo = Fusarium oxysporum; Foc = Fusarium oxysporum f. sp. conglutinans; Vd = Verticillium dahliae; Foe = Fusarium oxysporum f. sp. erythroxyli; Onu = Ophiostoma novo-ulmi; At = Arabidopsis thaliana; Nb = Nicotiana benthamiana; Avr = avirulence; I = immunity; LysM = lysin motif; PNP = plant natriuretic peptide; ¹ Corresponding R gene between brackets.