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. 2015 Oct 10;16(10):23994–24010. doi: 10.3390/ijms161023994

Figure 4.

Figure 4

Reduction of acetyl-coenzyme A (acetyl-CoA) content is involved in the mitochondrial dysfunction-mediated histone hypoacetylation in PDT-derived variants. (A) Acetyl-CoA content of the A375 and A375/6A5 cells was determined using the PicoProbe™Acetyl-CoA Fluorometric Assay Kit. The mRNA expressions of MMP9 (B) and PEG1 (C) were determined in A375 and A375/6A5 cells treated with 20 mM sodium acetate. β-actin served as loading control; (D) using the scratch wound assay, migration distance (µm) was measured 24 h after treated with 20 mM sodium acetate; and (E) after a treatment with 20 mM sodium acetate, the A375 and A375/6A5 cells were allowed to invade through the Matrigel. Invasion of the A375/6A5 cells was normalized to that of parental A375 cells. **, p < 0.01.