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MMP9 and PEG1 were down-regulated in the A375 ρ0 cells. (A) A375 cells were cultured in a medium containing ethidium bromide (EtBr) for 20, 40, or 60 days, and transcription levels of mitochondrial genes were measured with RT-PCR using primers that specifically target cytochrome oxidase c III (COX3) and NADH dehydrogenase 6 (ND6). Similarly, expression of MMP9 (B) and PEG1 (C) was determined. β-actin served as loading control. *, p < 0.05; ***, p < 0.001.
