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. 2015 Oct 12;16(10):24011–24031. doi: 10.3390/ijms161024011

Figure 2.

Figure 2

FA inhibits FGFR1 binding with FGF1 and attenuates FGFR1 tyrosine kinase activity. (A) Inhibition of FGFR1 kinase activity by FA was analyzed. Data are expressed as the percentages of the vehicle control. Data are from three independent experiments and are the mean ± SD. n = 3; (B) Effect of FA on the binding of FGFR1 to immobilized FGF1. Data are from three independent experiments and are the mean ± SD. n = 3, * p < 0.05, ** p < 0.01 compared to the control; (C) Western blot analysis of effect of FA on phosphorylation of FGR1. HUVEC were pre-treated with FA followed by stimulation with FGF1 for 2 min; p-FGFR1Y154 and GAPDH were assayed; (D) Immunofluorescent staining analysis of the effect of FA on FGFR1 expression in HUVEC. Cells were treated with FA under FGF1. The green color represents detection of p-FGFR1Y154, while nuclei were counterstained with blue color using DAPI (the scale bar represents 50 μm).