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. 2015 Oct 12;16(10):24032–24047. doi: 10.3390/ijms161024032

Figure 1.

Figure 1

MiR-30a is Downregulated in Podocyte Injury. (A) RT-qPCR analysis of miR-30a expression levels in glomeruli from BALB/c mouse after Adriamycin (ADR; 10.5 mg/kg) injection for 4, 7, 11, 15 and 20 days (n = 3, mean ± SD); (B) Western blot analysis of Nephrin and Desmin expression levels in cultured MPC5 cells treated with ADR at different doses (2.5, 5.0 and 10.0 µg/mL); Quantitative analysis of Nephrin and Desmin protein levels after normalization with β-tubulin; (C) RT-qPCR analysis of miR-30a expression levels in cultured MPC5 cells treated with ADR (5.0 µg/mL) at different time points (0.5, 2, 6, 12 and 24 h); (D) RT-qPCR analysis of miR-30a expression levels in the glomeruli from one patient with focal segmental glomerulosclerosis (FSGS) and one patient with kidney rupture (Control). The relative amount of miR-30a was normalized to U6. All data are presented as means ± SD from three independent experiments. * p < 0.05, ** p < 0.01, indicate statistically significant and highly statistically significant differences, respectively.