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Binding of biotinylated VEGF165 (5 ng/mL) to NRP-1 was evaluated at increasing concentration of TPC–peptide conjugates (3 to 960 µM) (data points show the mean ± S.D., n = 3). (A) Binding of different DKPPR–TPC conjugates to NRP-1 receptor; (B) Binding of different TKPRR–TPC conjugates with NRP-1 receptor. The different spacers were illustrated by colors: red for Ahx, green for PEG9 and blue for PEG18.
