Table 3.
Molar extinction coefficients of TPC and novel TPC–peptide conjugates.
| Compound | Molar Extinction Coefficient (L·M−1·cm−1) | Fluorescence and Singlet Oxygen Quantum Yields | |||||
|---|---|---|---|---|---|---|---|
| Soret | QIV | QIII | QII | QI | |||
| ε415 | ε516 | ε543 | ε598 | ε650 | Φf | Φ∆ | |
| TPC | 76.00 | 8.00 | 5.00 | 4.00 | 12.00 | 0.18 | 0.47 |
| TPC–Ahx–ATWLPPR | 74.00 | 6.80 | 4.90 | 2.60 | 12.00 | 0.18 | 0.39 |
| TPC–Ahx–DKPPR | 68.00 | 6.60 | 4.90 | 2.80 | 14.00 | 0.23 | 0.68 |
| TPC–PEG9–DKPPR | 92.80 | 7.40 | 5.60 | 3.20 | 13.00 | 0.30 | 0.70 |
| TPC–PEG18–DKPPR | 62.40 | 6.40 | 4.70 | 2.80 | 13.20 | 0.30 | 0.69 |
| TPC–Ahx–TKPRR | 73.00 | 4.50 | 3.50 | 2.20 | 10.00 | 0.22 | 0.70 |
| TPC–PEG9–TKPRR | 79.00 | 6.40 | 4.50 | 2.80 | 13.90 | 0.25 | 0.67 |
| TPC–PEG18–TKPRR | 88.30 | 6.20 | 4.30 | 2.40 | 14.90 | 0.27 | 0.67 |
Molar extinction coefficients determined at maximum wavelengths (ε)λ, fluorescence quantum yields (±0.02) (Φf) and singlet oxygen quantum yields (±0.05) Φ∆ of the conjugates. All measurements were performed at room temperature in ethanol. TPP solution in toluene was used as the fluorescence standard reference (Φf(ref) = 0.11) [24] while rose Bengal in ethanol was used as the standard reference for singlet oxygen quantum yields(Φ∆(ref) = 0.68) [25].