Figure 6.

GPR87 promotes cell proliferation and inhibits apoptosis via the p53 pathway in RT112 cells. The RT112 cells were collected and analyzed 5 days after infection with Ad-shGPR87 at a MOI of 20. (A) The p53 and its downstream signal p21 protein expression severely increased. On the other hand; (B) the MAPK pathway was not changed, but the PI3K/Akt pathway was decreased significantly. The gene expression (upper panel) and protein expression (lower panel) of p53 downstream gene p21 were also increased significantly in the wild-type p53 RT112 cells (C), but not in the mutant p53 HT1376 cells (D). Three independent experiments were performed for evaluating gene expression with real-time PCR or protein expression with western blot analyses. MOI: multiplicity of infection; ** p < 0.005, vs. Ad-scramble treatment; NS, not significant.