Table 2.
Microfluidics-based screening platforms described in this review.
| Protein | Experiment | Compartment | Result | Throughput (Events/s) |
|---|---|---|---|---|
| Horse radish peroxidase [65] | Mutagenesis (epPCR and saturation mutagenesis) | Yeast display, drop-based microfluidic system | 7-fold increase in catalytic efficiency towards Amplex Ultrared (AUR) | 2000 |
| Pseudomonas aeruginosa arylsulfatase [66] | Mutagenesis (epPCR) | Cell lysate, drop-based microfluidic system | 6-fold increase in promiscuous hydrolytic activity towards the nonnative substrate phosphonate | 926 |
| Streptomyces sp. β-glucosidase [71] | Mutagenesis (epPCR) | Cell lysate, drop-based microfluidic system combined with high-throughput DNA sequencing | 5.3 °C increase in thermostability | >100 |
| β-galactosidase [68] | Enrichment | In vitro transcription and translation, drop-based microfluidic system | 502-fold enrichment of positive variants from a mixture of active and inactive variants | 2000 |
| Yeast strain MH34α-amylase [69] | Mutagenesis (UV irradiation) | Whole cells, drop-based microfluidic system | 2-fold increase in α-amylase production | 323 |
| Cellulases for the hydrolysis of cellulosic biomass [79] | Metagenomics | Whole cells, drop-based microfluidic system | Identification of microorganisms with 17-fold higher cellobiohydrolase activity and 7-fold higher endogluconase activity | 6667 |