Skip to main content
. 2015 Oct 23;16(10):25502–25515. doi: 10.3390/ijms161025502

Figure 4.

Figure 4

Inhibitory effect of Fab on cytokine transcription in LPS-stimulated mouse macrophages. Humanized anti-TLR4 antibody Fab inhibits the cytokine transcription, including TNF-α (A); IFN-β (B); IL-6 (C); IL-1 (D), in mouse macrophages stimulated by LPS. Mouse macrophages were treated with humanized anti-TLR4 antibody for 2 h, and then stimulated with 1 µg/mL LPS for 4, 8, or 12 h. After stimulation the cells were collected, and TNF-α, IFN-β, IL-1, IL-6 were measured by Q-PCR. L: LPS; A: humanized anti-TLR4 antibody. Data are shown as mean ± SD (n = 6, * p < 0.05, ** p < 0.01 compared to LPS group).