Fig. 1.

BCL-xL is a direct target of miR-377. a BCL-XL expression, as determined by quantitative RT-PCR in ABT-199-resistant (ABT-199R) OC-R and S-6R relative to those in parental OCI-LY19 (OC) and SU-DHL-6 (S6) and cell lines. b RT-PCR analysis of BCL-xL and miR-377 expression levels in: parental OC, S6, and ABT-199R derivative OC-R and S6-R cells. c Schematic representation of firefly luciferase reporter constructs containing the 384-nucleotide sequence from the BCL-xL 3’-UTR and the corresponding binding regions for miR-377. d miR-377 target sequence base pairing in BCL-xL 3’-UTR. e Schematic of the mutant constructs. f Luciferase reporter activity in CHO-K1 cells co-transfected with BCL-xL WT-3’-UTR or MUT-3’-UTR constructs: mutants C1, C2, and C3 (double-mutant) and miR-377 or negative control (NC) mimics (10 nM) as indicated. g BCL-xL levels were assessed by immunoblot in 293 T cells transfected with miR-377 mimic at two concentrations (50 and 100 nM). β-actin was used as a loading control