Fig. 4.

Five miRNAs in the 14q32.31-32 clusters are coordinately down regulated in CLL patients. a Expression of five miRNAs, as determined by quantitative RT-PCR in CLL patients. Each dot represents one CLL patient. miRNA expression for each transcript was normalized to that of lymphocytes isolated from healthy donors as indicated by the strait line (corresponding to value 1). Significance was determined by a t-test. Expression levels of five miRNAs in treated and untreated CLL patients for b miR-127, c miR-136, d miR-154, e miR-337, and f miR-379. g Expression of five miRNAs in 14q32.31 and 14q32.32 clusters, as determined by quantitative RT-PCR in ABT-199R cell lines. miRNA expression for each transcript was normalized to that obtained in lymphocytes isolated from healthy donors, as indicated by the strait line