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. 2015 Oct 27;3(10):e12594. doi: 10.14814/phy2.12594

Figure 5.

Figure 5

Effects of p38 MAPK silencing on System A transport activity. Primary human trophoblasts were transfected with p38 MAPK siRNA or scrambled siRNA controls. (A) p38 MAPK protein expression in Scr siRNA or p38 siRNA-transfected cells at 90 h of culture. Data are presented as mean + SEM, n = 4. (B) Na+-dependent MeAIB uptake (System A) activity was measured in Scr siRNA or p38 siRNA-transfected cells with/without TNF-α (10 pg/mL) treatment. Data are presented as mean + SEM, n = 5. *P < 0.05, **P < 0.01. Cnt, control; TNF-α, tumor necrosis factor-alpha; Scr, scramble; p38, p38 MAPK.