Figure 1.

Schema of experimental design. Thirty-six mice were divided into three groups: control (Con), endurance training (END), and high-intensity interval training (HIIT). END and HIIT groups performed each training protocol for 6 weeks. Twenty-four hours after last training session, half mice of the each group performed performance test (PT). The mice swam to exhaustion with a load of 9% body weight to their tails. Immediately after PT, blood samples were collected for analysis of lactate and ammonia. Tibials anterior were also collected for muscle glycogen analysis. Not undergoing PT mice (remain of each group) were killed to collect blood samples for sedentary state ammonia and skeletal muscle for western blot and muscle glycogen in the same day.