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. 2015 Oct 27;3(10):e12597. doi: 10.14814/phy2.12597

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© 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Apoptosis in the soleus and plantaris muscles were determined by the terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay. (A) representative images of sections stained using the TUNEL assay. Soleus and plantaris muscles cross sections were immunoreacted with TUNEL-positive nuclei (red), DAPI (blue), Laminin (green) and a composite is depicted in Figure2A. TUNEL-positive interstitial cells were observed in soleus muscles (arrow). Bar in the picture is 50 μm. TUNEL-positive nuclei after 7 days of hindlimb unloading in the soleus (B) and plantaris (C) muscles. n = 6 per group. *P < 0.05 versus CT; ^†P < 0.05 versus HU; ^‡P < 0.05 versus HU + IWB.