Effect of downregulation and overexpression of TcPho91 on PPi, short and long-chain polyP levels. Extracts from TcPho91-OE (OE), or TcPho91 knockdown (KD) mutants showed increase or reduction in PPi (A), and short chain polyP (B) content, respectively, with no significant changes in long chain polyP content (C), as compared to the parental cell line (WT). Values are means ± SD of three different experiments. *Differences are statistically significant as compared to respective controls, p < 0.05 (Student’s t test).