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. Author manuscript; available in PMC: 2015 Nov 4.
Published in final edited form as: Biomater Sci. 2013 Jul 18;1(10):1101–1110. doi: 10.1039/C3BM60034C

Fig. 2.

Fig. 2

Effects of psw and wol scaffolds on hMSC activities. (a, h) Effects on attachment, growth, and proliferation. The hMSCs were seeded at a density of 10 000 cells cm−2 in growth medium; (f) effects on hMSC osteogenic induction as indicated by alkaline phosphatase (ALP) enzymatic activity normalized by the total number of cells (DNA). The hMSCs were seeded at a density of 30 000 cells cm−2 in osteogenic induction medium. The expression of ALP on psw at day 0 was below the detection limit. (a–f) LIVE (green)/DEAD (red) fluorescence microscopy images of hMSCs on psw (a, b, c) and wol (d, e, f) surfaces at days 0 (a, d), 8 (b, e) and 16 (c, f). (g) Total DNA and (h) total live cell number over time in growth medium. Results are reported after subtraction of cell-free blanks. Asterisks indicate statistically significant differences (p < 0.05).