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Primer sequences used for the RT-PCR
| Gene | Sequence 5′ to 3′ | |
|---|---|---|
| Osteocalcin | Forward | GGCAGCGAGGTAGTGAAGAG |
| Reverse | CTGGAGAGGAGCAGAACTGG | |
| Osteopontin | Forward | TGAAACGAGTCAGCTGGATG |
| Reverse | TGAAATTCATGGCTGTGGAA | |
| Runx2 or Cbfα-1 | Forward | CACCGAGACCAACAGAGTCA |
| Reverse | TGCTTGCAGCCTTAAACTGA | |
| β-Actin | Forward | GGACTTCGAGCAAGAGATGG |
| Reverse | AGCACTGTGTTGGCGTACAG | |
