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. 2015 Oct 30;4(5):10. doi: 10.1167/tvst.4.5.10

Figure 4.

Figure 4

ROS processing was blocked by antibody to αVβ5 integrins in ARPE-19 cells. Addition of 50 μg/mL of anti-αVβ5 antibody to media containing ROS for 3 hours decreased levels of ROS binding and ingestion in ARPE-19 cells (ROS + αVβ5) relative to samples devoid of blocking antibody (910.9 ± 114.8 vs. 2925.6 ± 120.8 arbitrary units, respectively; P < 0.001 [P = 1.4 × 10−13]). Similarly, addition of blocking antibody with ROS to ARPE-19 cells on NIT-treated samples (NIT + ROS + αVβ5) decreased levels of ROS binding and ingestion by 90% when compared with samples (NIT + ROS) devoid of antibody (215.0 ± 17.3 vs. 1825.1 ± 177.2 arbitrary units, respectively; P < 0.001 [P = 2.7 × 10−9]).