Table 2. Model parameters.

Parameter	Value	Note
$k_b^0$	0.00055nM −1 s −1	From Desai et al. 2015
k D	350s −1	From Walcott et al. 2012
k T	2μM −1 s −1	From Walcott et al. 2012
$\mathcal{C}$	70	From Walcott 2013, adjusted for closer myosin spacing
ε, pCa 7	0.02	From fits
ε, pCa 6	0.06	From fits (see S1 Supplementary Material)
ε, pCa 5	0.4	From fits
σ F	0.22 (scaled intensity)	Estimated from Desai et al. 2015 (see S1 Supplementary Material)
σ N	0.21–0.36 (scaled intensity)	Estimated from Desai et al. 2015, see Table 1
e	450 fluorescent units/s	Estimated from Desai et al. 2015 (see S1 Supplementary Material)
σ GFP	160 nm (2 pixels)	From Desai et al. 2015

Note: scaled intensity, defined in the text, gives a single fluorescently-labeled myosin an intensity of 1.