Table 1. Primer sequences used for Reverse Transcriptase—Polymerase Chain Reaction and sequencing of partial segment S1 of piscine reovirus.

Ta is the annealing temperature used for each set of primers.

Name	Sequences	Positions	Direction	Ta (°C)	Source
PRV-S1-F1	GATAAAGACTTCTGTACGTGAAAC	1–24	Forward	54	Kibenge et al [10]
PRV-S1-R1	GATGAATAAGACCTCCTTCC	1,062–1,081	Reverse	54	Kibenge et al [10]
S1PRV14F	GTACGTGAAACCCAAATGGCG	14–34	Forward	62	This study
S1PRV295R	CCAATCTCCGGGTGCAGAAT	276–295	Reverse	62	This study
S1PRV800F	CGAAGGATGTGCTATTCAGGC	800–820	Forward	62	This study
S1PRV1070R	CCTCCTTCCCCCTCAGAC	1,053–1,070	Reverse	62	This study
S1PRV287F1	GGAGATTGGACATGGAGTG	287–305	Forward	54	This study
S1PRV542R1	GATTTCAACTCGTCAGGG	535–552	Reverse	54	This study
S1PRV273F2	ATCATTCTGCACCCGGAG	273–290	Forward	54	This study
S1PRV569R2	TTGAATAGTCAGGGAAAGA	551–569	Reverse	54	This study
S1PRV541F	CGAGTTGAAATCTTTCCCTG	541–560	Forward	54	This study
S1PRV838R	GATGATGTTGGCTGGCATGCC	818–838	Reverse	54	This study
S1PRV541F	CGAGTTGAAATCTTTCCCTG	541–560	Forward	62	This study
S1PRV840R	TTGATGATGTTGGCTGGC	823–840	Reverse	62	This study
S1PRV270F	GGGATCATTCTGCACCCGG	270–288	Forward	54	This study
S1PRV562R	GTCAGGGAAAGATTTCAACTCG	541–562	Reverse	54	This study
S1PRV287F1	GGAGATTGGACATGGAGTG	287–305	Forward	54	This study
S1PRV569R2	TTGAATAGTCAGGGAAAGA	551–569	Reverse	54	This study
S1PRV12F1	CCTCTGGAGACCTTGGCTTG	495–514	Forward	60	This study
S1PRV244R	CAGGTACATCGCGATGGGAA	708–727	Reverse	60	This study
S1PRV25F	CCGCAAATCAGTTCCCACAC	657–676	Forward	60	This study
S1PRV238R	ATTCCAGAGAGCGCCATTCC	818–838	Reverse	60	This study
S1PRV56F	CATTGAAGCTAAGCGACGCC	439–458	Forward	60	This study
S1PRV246R	CAGTCTTCTGCTCCACTGGG	610–629	Reverse	60	This study
S1PRV12F2	ATCAATGCGATGGCGAAACG	246–286	Forward	60	This study
S1PRV224R	GGCGTCGCTTAGCTTCAATG	708–727	Reverse	60	This study
S1PRV33F	TGTGGGATCATTCTGCACCC	267–286	Forward	60	This study
S1PRV6F	CATTGAAGCTAAGCGACGCC	439–458	Forward	60	This study
S1PRV197R	CCAGTCTTCTGCTCCACTGG	611–630	Reverse	60	This study