Table 1. Primer sequences used for Reverse Transcriptase—Polymerase Chain Reaction and sequencing of partial segment S1 of piscine reovirus.
Name | Sequences | Positions | Direction | Ta (°C) | Source |
---|---|---|---|---|---|
PRV-S1-F1 | GATAAAGACTTCTGTACGTGAAAC | 1–24 | Forward | 54 | Kibenge et al [10] |
PRV-S1-R1 | GATGAATAAGACCTCCTTCC | 1,062–1,081 | Reverse | 54 | Kibenge et al [10] |
S1PRV14F | GTACGTGAAACCCAAATGGCG | 14–34 | Forward | 62 | This study |
S1PRV295R | CCAATCTCCGGGTGCAGAAT | 276–295 | Reverse | 62 | This study |
S1PRV800F | CGAAGGATGTGCTATTCAGGC | 800–820 | Forward | 62 | This study |
S1PRV1070R | CCTCCTTCCCCCTCAGAC | 1,053–1,070 | Reverse | 62 | This study |
S1PRV287F1 | GGAGATTGGACATGGAGTG | 287–305 | Forward | 54 | This study |
S1PRV542R1 | GATTTCAACTCGTCAGGG | 535–552 | Reverse | 54 | This study |
S1PRV273F2 | ATCATTCTGCACCCGGAG | 273–290 | Forward | 54 | This study |
S1PRV569R2 | TTGAATAGTCAGGGAAAGA | 551–569 | Reverse | 54 | This study |
S1PRV541F | CGAGTTGAAATCTTTCCCTG | 541–560 | Forward | 54 | This study |
S1PRV838R | GATGATGTTGGCTGGCATGCC | 818–838 | Reverse | 54 | This study |
S1PRV541F | CGAGTTGAAATCTTTCCCTG | 541–560 | Forward | 62 | This study |
S1PRV840R | TTGATGATGTTGGCTGGC | 823–840 | Reverse | 62 | This study |
S1PRV270F | GGGATCATTCTGCACCCGG | 270–288 | Forward | 54 | This study |
S1PRV562R | GTCAGGGAAAGATTTCAACTCG | 541–562 | Reverse | 54 | This study |
S1PRV287F1 | GGAGATTGGACATGGAGTG | 287–305 | Forward | 54 | This study |
S1PRV569R2 | TTGAATAGTCAGGGAAAGA | 551–569 | Reverse | 54 | This study |
S1PRV12F1 | CCTCTGGAGACCTTGGCTTG | 495–514 | Forward | 60 | This study |
S1PRV244R | CAGGTACATCGCGATGGGAA | 708–727 | Reverse | 60 | This study |
S1PRV25F | CCGCAAATCAGTTCCCACAC | 657–676 | Forward | 60 | This study |
S1PRV238R | ATTCCAGAGAGCGCCATTCC | 818–838 | Reverse | 60 | This study |
S1PRV56F | CATTGAAGCTAAGCGACGCC | 439–458 | Forward | 60 | This study |
S1PRV246R | CAGTCTTCTGCTCCACTGGG | 610–629 | Reverse | 60 | This study |
S1PRV12F2 | ATCAATGCGATGGCGAAACG | 246–286 | Forward | 60 | This study |
S1PRV224R | GGCGTCGCTTAGCTTCAATG | 708–727 | Reverse | 60 | This study |
S1PRV33F | TGTGGGATCATTCTGCACCC | 267–286 | Forward | 60 | This study |
S1PRV6F | CATTGAAGCTAAGCGACGCC | 439–458 | Forward | 60 | This study |
S1PRV197R | CCAGTCTTCTGCTCCACTGG | 611–630 | Reverse | 60 | This study |