Skip to main content
. 2015 Nov 4;10(11):e0142091. doi: 10.1371/journal.pone.0142091

Fig 1. Preparation of Inpp4a-deficient cells.

Fig 1

(A) Inpp4a mRNA was detected by RT-PCR with primer pairs shown in S1 Table. (B) Total RNAs from the mouse heart and Raw264.7 were subjected to RT-PCR. (C) Two lines of shInpp4a cells (seq1 and seq2) were prepared with the respective target sequences. The Inpp4a protein level was analyzed by western blotting. (D) Peritoneal macrophages were prepared from wild type (WT) or Inpp4a knockout (KO) mice. The Inpp4a protein level was determined by western blotting.