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. 2015 Nov 4;10(11):e0142091. doi: 10.1371/journal.pone.0142091

Fig 7. Timing of recruitment of Inpp4a and PtdIns(3)P during phagosome formation.

Fig 7

(A, B) RAW264.7 cells were transfected with mCherry-Inpp4a along with EGFP-[3×FYVE (EEA1)]. The cells were incubated with E-IgG at 37°C under a microscope. (B) The fluorescence intensity of each probe was quantified as described under "Materials and Methods" and shown as % of respective maximum values. The time course of Inpp4a was obtained as in Fig 5B, while that of PtdIns(3)P was calculated from 9 cells that were transfected with both mCherry-Inpp4a and EGFP-[3×FYVE (EEA1)]. Time zero in the figures indicates the time when the mCherry-Inpp4a fluorescence around E-IgG peaked.