Figure 4. WWP2 is important for regulating mitotic progression.

HeLa cells transfected with WWP2 siRNAs (siWWP2) or luciferase siRNA (siLuc) for one day were treated with nocodazole for 14 h. Mitotic cells collected by shake-off were released into the cell cycle. At various times post release, cells were collected and fractionated into cytoplasmic/soluble and chromatin-bound fractions. Equal amounts of lysates from both fractions were blotted for WWP2, cyclin A, cyclin B, phospho-H3, and β-actin.