Fig 3. Homeostatic response to sleep loss in Alk mutants.

A) Sleep profiles for the baseline, deprivation and recovery day were plotted against each other to show sleep deprivation and sleep rebound. The experiment was conducted entirely at 29°C. Iso31 and Alk ^ts similarly show an increase in sleep in the morning following sleep deprivation. B) Time course of recovery of sleep lost following deprivation. C) Minutes of sleep recovered on the first and second recovery day were compared between iso31 and Alk ^ts flies. 2-tailed Student’s t-test shows no significant difference between iso31 and Alk ^ts flies. D) Sleep latency after deprivation on recovery day one. *p<0.05. N = 31 for iso31. N = 29 for Alk ^ts.