Table 1. Source populations for fish used in assay design and testing.
| Species | Souce | Purpose |
|---|---|---|
| Yellowstone cutthroat trout | Clear Cr (Idaho, USA) | Sequence data; assay testing |
| Barnes Cr (Idaho, USA) | Sequence data; assay testing | |
| Goose Cr (Nevada, USA) | Sequence data; assay testing | |
| Cottonwood Cr, (Idaho, USA) | Sequence data; assay testing | |
| Upper Blackfoot R (Montana, USA) | Sequence data; assay testing | |
| Badger Cr (Idaho, USA) | Sequence data; assay testing | |
| Yellowstone L (Wyoming, USA) | Sequence data; assay testing | |
| Bonneville cutthroat trout | Bear L (Utah, USA) | Sequence data; assay testing |
| Bear R (Idaho, USA) | Sequence data; assay testing | |
| Glenwood FH (Utah, USA)* | Sequence data; assay testing | |
| Harkness Cr (Idaho, USA) | Sequence data; assay testing | |
| Rainbow trout | Skookumchuck R FH (Washington, USA)* | Sequence data |
| Gulkana R (Alaska, USA)* | Sequence data | |
| Dworshak FH (Idaho, USA)* | Sequence data | |
| Ennis FH—Arlee (Montana, USA)* | Assay testing | |
| Ennis FH—Eagle L (Montana, USA)* | Assay testing | |
| Ennis FH—Fish L (Montana, USA)* | Assay testing | |
| Ennis FH—Shasta (Montana, USA)* | Assay testing | |
| Ennis FH—McConaughy (Montana, USA)* | Assay testing | |
| Dry Cr (Idaho, USA) | Assay testing | |
| Sawtooth FH (Idaho, USA)* | Assay testing | |
| Shack Cr (Idaho, USA) | Assay testing | |
| Wallowa FH (Idaho, USA)* | Assay testing | |
| Bobtail Cr (Montana, USA) | Assay testing | |
| Silver Butte Fisher R (Montana, USA) | Assay testing | |
| E.F. Lolo Cr (Montana, USA) | Assay testing | |
| McCormick Cr (Montana, USA) | Assay testing | |
| Red Canyon (Montana, USA) | Assay testing | |
| Westslope cutthroat trout | John Day R (Oregon, USA) | Sequence data; assay testing |
| NF Elkhorn Cr (Idaho, USA) | Sequence data; assay testing | |
| Split Cr (Idaho, USA) | Sequence data; assay testing | |
| Withington Cr (Idaho, USA) | Sequence data; assay testing | |
| Duck Cr (Idaho, USA) | Sequence data; assay testing | |
| Heller Cr (Idaho, USA) | Sequence data; assay testing | |
| Rampike Cr (Idaho, USA) | Sequence data; assay testing | |
| Ditch Cr (Idaho, USA) | Sequence data; assay testing | |
| Crooked Fork Cr (Idaho, USA) | Sequence data; assay testing | |
| Pete King Cr (Idaho, USA) | Sequence data; assay testing | |
| Osier Cr (Idaho, USA) | Sequence data; assay testing | |
| Albert Cr (Montana, USA) | Sequence data; assay testing | |
| Fourmile Cr (Montana, USA) | Sequence data; assay testing | |
| Youngs Cr (Montana, USA) | Sequence data; assay testing | |
| Wounded Buck Cr (Montana, USA) | Sequence data; assay testing | |
| Twentyfivemile Cr (Montana, USA) | Sequence data; assay testing | |
| Flat Cr (Idaho, USA) | Sequence data | |
| Meadow Cr (Idaho, USA) | Sequence data | |
| French Cr (Idaho, USA) | Sequence data | |
| Baldy Cr (Idaho, USA) | Sequence data | |
| Twisp R (Washington, USA) | Sequence data | |
| Buck Cr (Washington, USA) | Sequence data | |
| American R (Idaho, USA) | Sequence data | |
| Falls Cr (Washington, USA) | Sequence data | |
| Hungery Cr (Idaho, USA) | Sequence data | |
| Cayuse Cr (Idaho, USA) | Sequence data | |
| Indian Grave Cr (Idaho, USA) | Sequence data | |
| Gravey Cr (Idaho, USA) | Sequence data | |
| Beaver Cr (Montana, USA) | Sequence data | |
| Moose Cr (Montana, USA) | Sequence data | |
| Deer Cr (Montana, USA) | Sequence data | |
| EF Bull R (Montana, USA) | Sequence data | |
| Ketchikan Cr (Montana, USA) | Sequence data | |
| McGuire Cr (Montana, USA) | Sequence data | |
| Martin Cr (Montana, USA) | Sequence data | |
| McCabe Cr (Montana, USA) | Sequence data | |
| Miller Cr (Montana, USA) | Sequence data | |
| Norton Cr (Montana, USA) | Sequence data | |
| Ontario Cr (Montana, USA) | Sequence data | |
| North Cr (Montana, USA) | Sequence data | |
| Tyler Cr (Montana, USA) | Sequence data | |
| Wilkes Cr (Montana, USA) | Sequence data | |
| Canuck Cr (Idaho, USA) | Sequence data | |
| Ball Cr (Idaho, USA) | Sequence data | |
| West Gold Cr (Idaho, USA) | Sequence data | |
| Beaver Cr (Idaho, USA) | Sequence data | |
| Mokins Cr (Idaho, USA) | Sequence data | |
| NF St. Joe R (Idaho, USA) | Sequence data | |
| Skin Cr (Idaho, USA) | Sequence data | |
| Slowey Cr (Montana, USA) | Sequence data | |
| Dry Wolf Cr (Montana, USA) | Sequence data | |
| Tributary of Armstrong Cr (Idaho, USA) | Sequence data | |
| Bad Luck Cr (Idaho, USA) | Sequence data | |
| Cedar Cr (Washington, USA) | Sequence data | |
| Ninemile Cr (Washington, USA) | Sequence data | |
| Scotchman Gulch (Montana, USA) | Sequence data | |
| Kraft Cr (Montana, USA) | Sequence data | |
| Leiberg Cr (Idaho, USA) | Sequence data | |
| N. Grouse Cr (Idaho, USA) | Sequence data | |
| SF Red R (Idaho, USA) | Sequence data | |
| Yoosa Cr (Idaho, USA) | Sequence data | |
| Ross Cr (Montana, USA) | Sequence data | |
| EF Emerald Cr (Idaho, USA) | Sequence data | |
| Bitter Cr (B.C., Canada) | Sequence data | |
| Blairmore Cr (B.C., Canada) | Sequence data | |
| Crazy Cr (B.C., Canada) | Sequence data | |
| Hartley Cr (B.C., Canada) | Sequence data | |
| Monk Cr (B.C., Canada) | Sequence data | |
| Pack R (Idaho, USA) | Sequence data | |
| Sheep Cr (Montana, USA) | Sequence data | |
| Toby Cr (B.C., Canada) | Sequence data | |
| Truman Cr (Montana, USA) | Sequence data | |
| Twin Cr (Idaho, USA) | Sequence data | |
| Waiparous Cr (B.C., Canada) | Sequence data | |
| Werner Cr (Montana, USA) | Sequence data | |
| Bostwick Cr (Montana, USA) | Sequence data | |
| Avalanche Cr (Montana, USA) | Sequence data | |
| Fish Cr (Montana, USA) | Sequence data | |
| NF Dupuyer Cr (Montana, USA) | Sequence data | |
| Sawmill Cr (Montana, USA) | Sequence data | |
| Fourmile Cr (Montana, USA) | Sequence data | |
| NF Teton R (Montana, USA) | Sequence data | |
| Thayer Cr (Montana, USA) | Sequence data | |
| Buffalo Cr (Montana, USA) | Sequence data | |
| Bluff Cr (Idaho, USA) | Sequence data | |
| Trail Cr (Idaho, USA) | Sequence data | |
| Floodwood Cr (Idaho, USA) | Sequence data | |
| Jacobs Ladder Cr (Idaho, USA) | Sequence data | |
| Blackbird Cr (Idaho, USA) | Sequence data | |
| Mill Cr (Idaho, USA) | Sequence data | |
| Morse Cr (Idaho, USA) | Sequence data | |
| MF Little Timber Cr (Idaho, USA) | Sequence data | |
| Big Cr (Idaho, USA) | Sequence data | |
| Boundary Cr (Idaho, USA) | Sequence data | |
| Colson Cr (Idaho, USA) | Sequence data | |
| Warm Springs Cr (Idaho, USA) | Sequence data | |
| Chinook salmon | SF Salmon R (Idaho, USA)* | Assay testing |
| Pahsimeroi R (Idaho, USA)* | Assay testing | |
| Clearwater R (Idaho, USA)* | Assay testing | |
| Coho salmon | Nez Perce Tribal FH (Idaho, USA)* | Assay testing |
| Sockeye salmon | Redfish L (Idaho, USA)* | Assay testing |
Locations marked with an asterisk are not shown in Fig 2. Sequencing; sequences from fish were used for initial assay design, Assay testing; extracted tissue samples from fish were used for assay testing.