Table 3. Summary of results of the validated taxon-specific qPCR assays.
Assay | Problematic polymorphism |
---|---|
Yellowstone cutthroat | Bonneville cutthroat trout also amplify |
westslope cutthroat | Rare polymorphism caused some Youngs Cr individuals not to amplify |
rainbow trout | Delayed amplification for individuals from Eagle Lake (O. mykiss aquilarium) |
Except for the noted problematic polymorphisms, each assay was taxon-specific and amplified all individuals of the target taxon with similar efficiency.