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(A) KNP74(ura4 +), and KNP95 (Δura4) cells expressing Fur4-GFP were grown in YES liquid medium for 12 h. Cells were washed twice with sterile water and suspended in EMM, EMMU, EMM(-N), or EMMU(-N) media and incubated at 30°C for 1 h. Cells were observed using fluorescence microscopy. Bar: 10 μm.
